Human Cytomegalovirus Infection Suppresses CD34(+) Progenitor Cell Engraftment in Humanized Mice
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Author
Crawford, Lindsey BTempel, Rebecca
Streblow, Daniel N
Yurochko, Andrew D
Goodrum, Felicia D
Nelson, Jay A
Caposio, Patrizia
Affiliation
Univ Arizona, BIO5 Inst, Dept ImmunobiolIssue Date
2020-04-06Keywords
Hematopoiesishematopoietic stem cell transplant
Human Cytomegalovirus
humanized mice
myelosuppression
progenitor cell
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Crawford, L.B.; Tempel, R.; Streblow, D.N.; Yurochko, A.D.; Goodrum, F.D.; Nelson, J.A.; Caposio, P. Human Cytomegalovirus Infection Suppresses CD34+ Progenitor Cell Engraftment in Humanized Mice. Microorganisms 2020, 8, 525.Journal
MICROORGANISMSRights
Copyright © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).Collection Information
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.Abstract
Human cytomegalovirus (HCMV) infection is a serious complication in hematopoietic stem cell transplant (HSCT) recipients due to virus-induced myelosuppression and impairment of stem cell engraftment. Despite the clear clinical link between myelosuppression and HCMV infection, little is known about the mechanism(s) by which the virus inhibits normal hematopoiesis because of the strict species specificity and the lack of surrogate animal models. In this study, we developed a novel humanized mouse model system that recapitulates the HCMV-mediated engraftment failure after hematopoietic cell transplantation. We observed significant alterations in the hematopoietic populations in peripheral lymphoid tissues following engraftment of a subset of HCMV+ CD34(+) hematopoietic progenitor cells (HPCs) within the transplant, suggesting that a small proportion of HCMV-infected CD34(+) HPCs can profoundly affect HPC differentiation in the bone marrow microenvironment. This model will be instrumental to gain insight into the fundamental mechanisms of HCMV myelosuppression after HSCT and provides a platform to assess novel treatment strategies.Note
Open access journalISSN
2076-2607PubMed ID
32268565Version
Final published versionae974a485f413a2113503eed53cd6c53
10.3390/microorganisms8040525
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Except where otherwise noted, this item's license is described as Copyright © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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